Retinol transport system in cattle and purification of retinol-binding protein from bovine serum.
نویسندگان
چکیده
منابع مشابه
Confocal immunolocalization of bovine serum albumin, serum retinol-binding protein, and interphotoreceptor retinoid-binding protein in bovine retina.
PURPOSE Recently it has been shown that the transport as well as clearance of retinol from isolated rod photoreceptors requires an extracellular factor. Interphotoreceptor retinoid-binding protein (IRBP) is a component of the interphotoreceptor matrix (IPM) and is known to bind visual cycle retinoids. Serum albumin and serum retinol-binding protein (sRBP), proteins capable of binding retinoids,...
متن کاملUptake of retinol and retinoic acid from serum retinol-binding protein by retinal pigment epithelial cells.
Isolated pigment epithelial cells from bovine eyes were shown to be capable of taking up retinol and retinoic acid. The uptake was dependent on the ligands being presented to the cells as complexes with serum retinol-binding protein. Neither free retinol nor retinol or retinoic acid presented as serum albumin complexes were taken up by the pigment epithelial cells. The transport of retinal and ...
متن کاملBiochemical indicators of vitamin A deficiency: serum retinol and serum retinol binding protein.
Two biochemical indicators are currently recommended for determining whether vitamin A deficiency (VAD) is a public health problem: serum retinol and serum retinol-binding protein (RBP). After consideration of 40 data sets and the original rationale for previously proposed cut-offs, a cut-off for serum retinol concentration was proposed at <0.70 micro mol/L (20 micro g/dL) in > or =15% of the s...
متن کاملInteractions of plasma retinol-binding protein with its receptor. Specific binding of bovine and human retinol-binding protein to pigment epithelium cells from bovine eyes.
Bovine and human retinol-retinol-binding protein (RBP) were iodinated to high specific activity. At least 34% of the iodinated material was native and retained its retinol chromophore as judged by its characteristic ability to bind to plasma prealbumin. Bovine and human retinol-125I-RBP were found to bind specifically to intact isolated pigment epithelium cells from bovine eyes. The specific bi...
متن کاملPurification of retinol dehydrogenase from bovine retinal rod outer segments.
We purified retinol dehydrogenase from bovine rod outer segments using polyethylene glycol precipitation and hydroxylapatite, concanavalin A-Sepharose CL-4B, and Sepharose CL-6B column chromatography in the presence of NADP. We obtained 13-fold purification of retinol dehydrogenase with specific activity of 61.8 nmol/min/mg and 3.8% recovery. Sodium dodecyl sulfate-polyacrylamide gel electropho...
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ژورنال
عنوان ژورنال: The Japanese Journal of Veterinary Science
سال: 1989
ISSN: 0021-5295,1881-1442
DOI: 10.1292/jvms1939.51.408